Thermal Stability of Phaseolus vulgaris Leucoagglutinin: a Differential Scanning Calorimetry Study
نویسندگان
چکیده
منابع مشابه
Thermal stability of Phaseolus vulgaris leucoagglutinin: a differential scanning calorimetry study.
Phaseolus vulgaris phytohemagglutinin L is a homotetrameric-leucoagglutinating seed lectin. Its three-dimensional structure shows similarity with other members of the legume lectin family. The tetrameric form of this lectin is pH dependent. Gel filtration results showed that the protein exists in its dimeric state at pH 2.5 and as a tetramer at pH 7.2. Contrary to earlier reports on legume lect...
متن کاملUsing Differential Scanning Calorimetry in Understanding the Correlation Between Thermal Stability and Protein Stability: A Case Study
During the lifetime of a therapeutic protein it will be subjected to different conditions during processing and storage that can often involve low pH, different buffer components and ionic strengths, and thermal fluctuations during storage and transportation. In addition, it must be stable in the final formulation for several years, often at very high protein concentrations. The final drug prod...
متن کاملDifferential Scanning Calorimetry - A Method for Assessing the Thermal Stability and Conformation of Protein Antigen.
Differential scanning calorimetry (DSC) is an analytical technique that measures the molar heat capacity of samples as a function of temperature. In the case of protein samples, DSC profiles provide information about thermal stability, and to some extent serves as a structural "fingerprint" that can be used to assess structural conformation. It is performed using a differential scanning calorim...
متن کاملEfferent projections from the subnucleus reticularis dorsalis (SRD): a Phaseolus vulgaris leucoagglutinin study in the rat.
Small iontophoretic applications of Phaseolus vulgaris leucoagglutinin (PHA-L) were used in rats to study the efferent projections from a restricted region in the reticular formation of the caudal medulla: the subnucleus reticularis dorsalis (SRD). It was found that fibres from SRD project mainly to the spinal cord, the oral motor nuclei, the dorsal accessory inferior olive, the giganto- and pa...
متن کاملThe thermal stability and domain interactions of the mannitol permease of Escherichia coli. A differential scanning calorimetry study.
The thermal stability and domain interactions in the mannitol transporter from Escherichia coli, enzyme IImtl, have been studied by differential scanning calorimetry. To this end, the wild type enzyme, IICBAmtl, as well as IICBmtl and IICmtl, were reconstituted into a dimyristoylphosphatidylcholine lipid bilayer. The changes in the gel to liquid crystalline transition of the lipid indicated tha...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BMB Reports
سال: 2002
ISSN: 1976-6696
DOI: 10.5483/bmbrep.2002.35.5.472